![]() ![]() Glycine was the most predominant amino acid. The maps were also different from those of type I collagen from calf skin, suggesting the differences in amino acid sequences between both collagens. ![]() Different peptide maps were observed between ASC hydrolyzed by V8 protease and lysyl endopeptidase. ASC with the yield of 4.19% (wet weight basis) was identified as type I collagen, which was composed of two α1 chains and one α2 chain. Therefore, gelatins from the skin of BBS has a potential to replace mammalian for gelatin, due to its similarity in bloom strength and setting behavior to the commercial bovine bone gelatin.Īcid solubilized collagen (ASC) was extracted from the skin of unicorn leatherjacket (Aluterus monoceros) using 0.5 M acetic acid, followed by precipitation with 2.6 M NaCl. Fourier transform infrared (FTIR) study revealed that the major absorption bands of gelatin from the skins of both sharks shifted to a higher wavenumber, compared with their corresponding acid soluble collagen (ASC). Gelatin from BTS skin was more susceptible to hydrolysis than that from BBS skin. Cross-linked components (- and -chains) and -chains were more degraded with increasing extraction temperatures, especially at 75 ฐC. Scanning electron microscopic study showed that gelatin gel from BBS skin presented the thicker strand than those from BTS skin and bovine bone. However, gelatin gels from BTS skin was not able to set within 3 h at the same temperature. Gelatin gels from BBS skin could set at room temperature (25-26 ฐC) within 24 min. Gelatins from both species extracted at 45 C for 6 h exhibited the highest bloom strength (206-214 g), which was higher than that of commercial bovine bone gelatin (197 g) (p < 0.05). Yields of gelatin from the skins of BBS and BTS were 19.06-22.81% and 21.17-24.76% (based on wet weight), respectively. Gelatins from the skins of brownbanded bamboo shark (BBS Chiloscyllium punctatum) and blacktip shark (BTS Carcharhinus limbatus) were extracted using the distilled water at different temperatures (45, 60 and 75 ฐC) and times (6 and 12 h). ![]()
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